Open AccessSurvei Entomologi, Kerentanan Aedes aegypti terhadap Insektisida Organofosfat, dan Identifikasi Gen VGSC di Dusun Malangrejo, Sleman, Yogyakarta
Author(s) -
Novyan Lusiyana,
Siti Fitiah,
Andrias Atmaja Putri,
Muthia Tsabita Rahmi,
Dian Maknalia Ilham
Publication year - 2019
Publication title -
aspirator
Language(s) - English
Resource type - Journals
eISSN - 2338-7343
pISSN - 2085-4102
DOI - 10.22435/asp.v11i1.438
Subject(s) - aedes aegypti , malathion , organophosphate , dengue fever , biology , toxicology , pyrethroid , insecticide resistance , larva , microbiology and biotechnology , pesticide , virology , ecology
Control and prevention effort of dengue haemorhagic fever relies on the application of insecticidesto control vector mosquito. In Indonesia, application of organophosphate insecticide (temephos andmalathion), and pyrethroid is a key part of the dengue control strategy. This study aimed to determine theentomological survey, susceptibility of Aedes aegypti against organophosphate insecticide and identificationof VGSC gene in Dusun Malangrejo, Sleman Yogyakarta. This study is an observational with a cross sectionaldesign. The entomology survey procedure used a single larvae technique, biological susceptibility test of Ae.aegypti against organophosphate insecticides using WHO (temephos), CDC (malathion), biochemistry andVGSC genes detection using PCR method. Results of this study showed that bath tub was potential breedingplace for Ae. aegypti. Susceptibility status of temephos was still tolerant (95.4%), while malathion showsresistance (13.75%). Bioassay and biochemical test shows potential resistant to insecticides due to elevatedactivity of esterase non specific enzyme. Detection of the VGSC gene shows positive for both genes (V1016G;S989P) and F1534C. Furthermore, regularly assessment of insecticide resistance status will hopefully assistthe local public health authorities to assign policy for managing mosquito control such as by rotation ofdifferent routinely used insecticide to delay Ae. aegypti resistance development.