Open AccessDetermination of Sulfamethoxazole in Pure and Pharmaceutical Samples by Using Direct Method for Calibration Curve of Normal Spectrum of UV-Spectrophotometry
Author(s) -
Sarra A. Abrahem,
Khaleda H. Al-Saidi
Publication year - 2020
Publication title -
al-nahrain journal of science
Language(s) - English
Resource type - Journals
eISSN - 2663-5461
pISSN - 2663-5453
DOI - 10.22401/anjs.23.4.01
Subject(s) - calibration curve , chromatography , correlation coefficient , analytical chemistry (journal) , linearity , calibration , spectrophotometry , sulfamethoxazole , mathematics , chemistry , detection limit , statistics , physics , antibiotics , quantum mechanics , biochemistry
Normal spectra for sulfamethoxazolesolutions were developed and used for the determination of sulfamethoxazole (SMX) antibiotic by using zero-crossing technique and simultaneously determining (SMX) at wavelength 259.00 nm. The correlation coefficient of the calibration curve for the normalspectrum was 0.9990. Linearity was maintained by using concentrations (0.990104M, 0.996104M, 0.999104M, 1.004104M, 1.005104M) and the percentage recovery of sulfamethoxazolesamples were (99.00%, 99.60%, 99.90 %, 100.40 % and 100.50 %). A statistical analysis confirmed, a precision with accuracy for simultaneous fixing of (SMX). In addition, a British pharmacopoeia method was compared with the method used in this paper using F test.