Open AccessProtective properties of biotextile indicated by immature gamete cells flow cytometry and Drosophila melanogaster survival tests
Author(s) -
Dace Grauda,
AUTHOR_ID,
Andra Miķelsone,
Dalius Butkauskas,
Dāvis Rašals,
Inese Vilcāne,
Valters Gobiņš,
Inga Lashenko,
AUTHOR_ID,
AUTHOR_ID,
AUTHOR_ID
Publication year - 2022
Language(s) - English
Resource type - Conference proceedings
DOI - 10.22364/iarb.2021.06
Subject(s) - drosophila melanogaster , melanogaster , helmholtz coil , flow cytometry , biology , biophysics , magnetic field , microbiology and biotechnology , physics , genetics , quantum mechanics , gene
The testing of an innovative amber-containing biotextile developed within the EUREKA project IFSITEX revealed the ability of biotextile material to protect test objects from UV-B radiation and extremely low-frequency electromagnetic fields (ELF-EMFs). One of two different testing methods is based on flow cytometry indicating changes in the relative fluorescence of immature plant gametes based on the determination of the sum of changes in approximately 20 various parameters of gametic cells affected in one nuclear stage by irradiation of cell suspension covered with biotextile fabrics containing amber particles or similar fabrics lacking amber particles with UV-B for 75 minutes. The second test called Drosophila melanogaster survivability test, specifically modified for biotextile testing, revealed differences in the percentage of successful development of D. melanogaster from larvae to imago stage after growing fruit flies in tubes covered with biotextile material containing amber particles as well as material free of amber particles. Tubes with D. melanogaster larvae were placed in a Helmholtz coil were continuously exposed to a sinusoidal 50 Hz magnetic field (MF) at a flux of 450 μT. Survivability results were compared to the success of the development of the control which was not affected by experimentally generated ELF-EMFs D. melanogaster. Samples of biotextile containing amber particles were found to retain UV-B and LF-EMF radiation better than fabric samples without amber particles.