Open AccessSpecific Identification Method based on PCR for Drosophila melanogaster
Author(s) -
Yousef Naserzadeh,
Elena Pakina,
Abdorreza Mohammadi Nafchi,
Anvar Gadzhikurbanov
Publication year - 2020
Publication title -
vestnik rossijskogo universiteta družby narodov. seriâ agronomiâ i životnovodstvo
Language(s) - English
Resource type - Journals
eISSN - 2312-7988
pISSN - 2312-797X
DOI - 10.22363/2312-797x-2020-15-2-134-141
Subject(s) - drosophila melanogaster , genbank , melanogaster , biology , polymerase chain reaction , genetics , gene , mitochondrial dna , drosophilidae , dna sequencing
D. melanogaster is one of the most harmful citrus fruit flies having a large number of host plants. The molecular diagnostic method has been created for identification the D. melanogaster from another non-quarantine species Drosophila spp. The proposed method for differentiation is to use the mitochondrial DNA cytochrome oxidase I gene region 709-bp. We amplified samples of DNA with primers Droso-S391 and Droso-A381 by D. melanogaster, D. suzukii, and D. Simulans collections in the laboratory samples from many countries and contrasted with sequences of other GenBank Drosophila taxa. The findings of a polymerase chain reaction (PCR) based on DNA sequence polymorphisms showed that these primers accurately identify the area of the gene as well as the unique primers of Drosophila melanogaster.