Open AccessSHORT PEPTIDES REGULATE THE GROW OF CALLUS CULTURE TOBACCO NICOTIANA TABACUM L
Author(s) -
Т. А. Диловарова,
Т. А. Диловарова,
С В Смесова,
С В Смесова,
Е. Н. Баранова,
Е. Н. Баранова,
L. I. Fedoreyeva,
Л.И. Федореева
Publication year - 2017
Publication title -
vestnik rossijskogo universiteta družby narodov. seriâ agronomiâ i životnovodstvo
Language(s) - English
Resource type - Journals
eISSN - 2312-7988
pISSN - 2312-797X
DOI - 10.22363/2312-797x-2017-12-4-323-331
Subject(s) - nicotiana tabacum , callus , explant culture , biology , nicotiana , peptide , regulator , gene , microbiology and biotechnology , botany , gene expression , in vitro , solanaceae , biochemistry
Phytohormones, which are secreted peptides, play an important role in intercellular inter-actions, participating in the regulation of development and in numerous physiological processes and in responses to the influence of environmental factors. Short peptides in a concentration of 10-7-10-8 M regulate the growth and development of the callus culture of tobacco (Nicotiana tabacum L.) in culture in vitro. AlaGluAspGly, AlaAspGluLeu, GlyGly and GlyAsp on the 28th day of cultivation showed an increase in the biomass of tobacco by a factor of 1.5 to 2.5, an increase in the number of regenerants per explant by 10-30%, and also the area of the leaf plate of regenerants by 2-2.5 times. Exogenous peptides influence the expression of genes encoding the proteins of growth factor regulators. It was found that the expression values calculated by the PCR-PB method depend on the nature of the peptide. In addition, structurally different peptides differentially affect the different genes (growth regulating factor) of GRF. The greatest increase in the expression level of GRF family genes is observed in the presence of AlaAspGluLeu - GRF1, GRF3, GRF4 3.5-4 times, in the presence of AlaGluAspGly - GRF2 more than 4.5 times, GlyGly - GRF4 more than 3 times, GlyAsp - GRF3, GRF4 in 3-4 times. It is assumed that in the cell culture, short peptides can act as a regulator of the growth of new generation plants that can find application in biotechnology and practical plant growing.