Open AccessSelection combinations of primers for determining intervarietal and intravarietal polymorphism Glycine hispida
Author(s) -
Е. В. Романова,
A.A. Kochumova,
Е.О. Shmelkova
Publication year - 2015
Publication title -
vestnik rossijskogo universiteta družby narodov. seriâ agronomiâ i životnovodstvo
Language(s) - English
Resource type - Journals
eISSN - 2312-7988
pISSN - 2312-797X
DOI - 10.22363/2312-797x-2015-2-16-23
Subject(s) - amplified fragment length polymorphism , primer (cosmetics) , biology , genotype , polymorphism (computer science) , genetics , genome , selection (genetic algorithm) , gene , genetic diversity , chemistry , artificial intelligence , computer science , population , demography , organic chemistry , sociology
The article describes the experience of selecting combinations of primers for AFLP-analysis intravarietal and intervarietal polymorphism soybean accessions. During the experiment, has been tested 18 primer combinations. As a result, were selected 5 primer combinations, the most informative labeling for the soybean genome. Total detect 132 AFLP-fragments, of which 60 were polymorphic and characterized by individual genotypes varieties.