Open AccessThe study of factors affecting the efficiency of somatic transgenesis in chickens
Author(s) -
Yuri Prytkov,
А. Н. Ветох,
Н. А. Волкова,
A.A. Nikishov,
N. A. Zinovieva
Publication year - 2014
Publication title -
vestnik rossijskogo universiteta družby narodov. seriâ agronomiâ i životnovodstvo
Language(s) - English
Resource type - Journals
eISSN - 2312-7988
pISSN - 2312-797X
DOI - 10.22363/2312-797x-2014-3-49-54
Subject(s) - transgenesis , oviduct , transformation (genetics) , somatic cell , biology , transgene , transformation efficiency , vector (molecular biology) , microbiology and biotechnology , genetics , embryo , gene , reproductive technology , recombinant dna , embryogenesis , endocrinology , agrobacterium
In this article we studied the impact of the use of retroviral vectors for genetic transformation of chicken oviduct cells in vivo to produce transgenic chickens bioreactors and investigated factors affecting the efficiency of local transgenesis in chickens. By using of retroviral vector system it was shown that with the efficiency of chickens oviduct cells transformation was at least 17,2 ± 3,1%.