Open AccessDevelopment diagnostic methods of bacterial blight of rice on based PCR
Author(s) -
М.А. Егорова,
А. Н. Игнатов,
Е. С. Мазурин
Publication year - 2014
Publication title -
vestnik rossijskogo universiteta družby narodov. seriâ agronomiâ i životnovodstvo
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.281
H-Index - 4
eISSN - 2312-7988
pISSN - 2312-797X
DOI - 10.22363/2312-797x-2014-2-22-27
Subject(s) - bacterial blight , xanthomonas oryzae pv. oryzae , blight , biology , pathogen , gentamicin , microbiology and biotechnology , cefazolin , cycloheximide , polymerase chain reaction , xanthomonas oryzae , antibiotics , horticulture , gene , genetics , protein biosynthesis
Primers identifying bacterial blight pathogen, without cross-reactions with closely related species were designed based on the analysis of the original and GeneBank DNA sequences of Xanthomonas oryzae pv. oryzae. Media YPGA containing cycloheximide 50 mg / l + cefazolin 30 mg / l + gentamicin 2 mg / l has been optimized for BIO-PCR. Sensitivity of the BIO-PCR analysis using original selective media up to 100 times more effective than direct PCR analysis. The sensitivity of the primers in BIO-PCR analysis with original selective media has achieved 50 CFU/ml.