Open AccessTHE ORETICAL BASIS AND EXPERIMENTAL STUDY OF COMPLEX OF MANGANESE (II) WITH HEXAMETHYL- PROPILENAMIN-OXIME AS PARAMAGNETIC CONTRAST AGENT FOR MR-IMAGING OF MALIGNANT TUMORS
Author(s) -
W. Yu. Ussov,
M. L. Belyanin,
В. Д. Филимонов,
М. Г. Данилец,
I. V. Milto,
Ж. В. Веснина,
М. А. Зоркальцев,
Miloš Lučić,
N. L. Shimanovskiy
Publication year - 2019
Publication title -
lučevaâ diagnostika i terapiâ
Language(s) - English
Resource type - Journals
eISSN - 2079-5351
pISSN - 2079-5343
DOI - 10.22328/2079-5343-2019-10-2-42-49
Subject(s) - manganese , nuclear medicine , chemistry , paramagnetism , nuclear magnetic resonance , medicine , physics , organic chemistry , quantum mechanics
Aim of the study . We attempted to obtain the Mn-HMPAO complex (Mangoxim) and to evaluate the possibility to apply as a paramagnetic contrast agent for enhancement of tumors at MRI. Materials and methods . The synthesis of HMPAO was carried out by using R. D. Neirinckx — J. Pijarowska-Kruszyna technique (2017), without separation of enantiomers. The Mn-HMPAO was obtained by mixing Manganese(II) carbonate with HMPAO, pH 6,2–6,5, 0,5 M. Also the quantum chemistry calculations were carried out on the structure of Mn-HMPAO using method of functional of density DFT rb3lyp/6–31+g(d,p) for isolated molecules and for water solutions (CPCM). MRI study of Mn-HMPAO uptake carried out in twelve laboratory mouses with transplanted Lewis sarcoma, in T1-w. SE mode, TR=500 ms, TE=15 ms, 256×256 matrix, FOV 15×15 cm, 3 mm thin slice. MRI acquired in 15–17 min and 60–90 min after intravenous injection of the Mn-HMPAO. The uptake was quantified as index of enhancement: IE=(Int. T1-w.MRI)Mn-HMPAO/(Int. T1- w.MRI)pre-contrast . Results . The N,N-Mn-dioxim is more stable than O,O-Mn-dioxim for 50,5 KCal/mol, so in the nature only N,N-Mn-dioxim exists. R1 of Mn-HMPAO was 3,35 1/(mM•s), below R1 of gadopentetate=3,96 1/(mM•s). The highest value of early IE was over the tumor (3,36±0,31) and over lungs (3,07±0,43). At 60 min the IE over tumor decreased to 1,58±0,14, remaining far over IE values over any other location. Conclusion . The Mn-HMPAO complex demonstrates paramagnetic properties for the application as the contrast MRI agent and probably will improve the selective enhancement of tumors.