Open AccessLiver proliferating cell nuclear antigen, BAX/Bcl-2 ratio, collagen, and polysaccharide accumulation as diagnostic tools in experimental hepatocellular carcinoma
Author(s) -
Nabil Mohie Abdel-Hamid,
Mohamed K. Hassan,
Amal AM Ahmed,
Sara Gamal Abd Allah,
Nahla Anber
Publication year - 2022
Publication title -
journal of contemporary medical sciences
Language(s) - English
Resource type - Journals
eISSN - 2415-1629
pISSN - 2413-0516
DOI - 10.22317/jcms.v8i1.1162
Subject(s) - malondialdehyde , proliferating cell nuclear antigen , hepatocellular carcinoma , glutathione , superoxide dismutase , oxidative stress , chemistry , apoptosis , ccl4 , antigen , pathology , medicine , endocrinology , immunohistochemistry , immunology , biochemistry , enzyme , carbon tetrachloride , organic chemistry
Objectives: This experimental study was conducted to look for a sensitive diagnostic panel for early detection of HCC.
Methods: Combination of diethyl nitrosamine (DENA, 200 mg/kg, IP, once), two weeks later, CCl4(3ml/kg/week), subcutaneously, for 6 weeks) induced HCC in rats. Sixteen male Wistar rats were divided into 2 groups, control, and HCC.
Results: DENA plus CCl4 elevated serum alpha-fetoprotein, liver enzyme activity and depressed superoxide dismutase, reduced glutathione (GSH), elevated malondialdehyde. Also, depressed caspase-3, elevated collagen, polysaccharide accumulation, proliferating cell nuclear antigen in liver tissues, and depressed BAX / Bcl-2 ratio.
Conclusion: Decreased BAX/Bcl-2 ratio, elevated collagen deposition, polysaccharide accumulation, nuclear proliferation, and tissue oxidative stress help in the early diagnosis of liver cancer.