Open AccessEremostachys Binalodensis, A Potential Therapeutic Choice for Gingival Inflammatory Wounds
Author(s) -
Armin S.Hariri,
Sevda Shayesteh,
Parina Asgharian,
Mohsen Chamanara,
Maryam-Sadat Sadrzadeh-Afshar
Publication year - 2021
Publication title -
journal of contemporary medical sciences
Language(s) - English
Resource type - Journals
eISSN - 2415-1629
pISSN - 2413-0516
DOI - 10.22317/jcms.v7i3.976
Subject(s) - wound healing , tumor necrosis factor alpha , stimulation , secretion , inflammatory response , medicine , pharmacology , scratching , inflammation , immunology , physics , acoustics
Objectives: In this study we aimed to evaluate the effect of E. binalodensis on gingival inflammatory wounds. Methods: In-vitro wound was induced by scratching the surface layer of human gingival fibroblasts (hGFs). Cells were pretreated with 1,10,100,1000 µg/ml of E. binalodensis methanol extract prior to 1µg/ml LPS stimulation. hGFs proliferation was assessed by MTT test. Also levels of critical inflammatory cytokines such as IL-1, IL-6 and TNF- were determined by enzyme-linked immunosorbent assay (ELISA). Results: Wound induction was associated with secretion of IL-1β, IL-6 and TNF-α from hGFs. E. binalodensis enhanced the hGFs proliferation besides reducing the level of IL-1β, IL-6 and TNF-α in LPS-scratch-stimulated hGFs. Conclusions: Regarding anti-inflammatory and proliferative effects of E. binalodensis on hGFs, availability and safety of it, it is suggested for enhancing the wound healing process in gingival inflammatory wounds.