Open AccessTriiodothyronine Improves Morphology and Up-regulates seladin-1 of neurospheres extracted from Subventricular Zone in Streptozotocin-induced rat model of Alzheimer's disease
Author(s) -
Tahmineh Mokhtari,
Simin Mahahakizadeh,
Hadi Aligholi,
Sahar Ijaz,
Leila Noori,
Gholamreza Hassanzadeh
Publication year - 2020
Publication title -
journal of contemporary medical sciences
Language(s) - English
Resource type - Journals
eISSN - 2415-1629
pISSN - 2413-0516
DOI - 10.22317/jcms.v6i1.735
Subject(s) - neurosphere , subventricular zone , streptozotocin , triiodothyronine , endocrinology , medicine , chemistry , biology , microbiology and biotechnology , diabetes mellitus , neural stem cell , stem cell , gene , biochemistry , cellular differentiation , hormone , adult stem cell
Objectives: In this study, the effects of triiodothyronine (T3) on neurospheres isolated from SVZ of AD induced rats were examined. Methods: Eighteen male Wistar rats were classified into two groups: Sham (Sh) and STZ (Streptozotocin injected, 1.5 mg/kg in each lateral ventricle on days 1 and 3 after recovery). On day 21, the SVZ was extracted and neurospheres were cultured. T3 (50 nM) was added to the culture medium (STZ+T3 group) and then, the morphology and seladin-1 gene expression of neurospheres were evaluated. Results: The diameter and the number of neurospheres along with the gene expression of seladin-1 were significantly decreased in the STZ group compared to Sh group (P˂0.05) while the administration of T3 significantly (P˂0.05) increased all these parameters in the STZ group. Conclusion: STZ decreases the proliferation of stem cells extracted from SVZ and administration of T3 to the culture media improves the morphology and up-regulates the gene expression of seladin-1 of neurospheres.