Histological impact of nutritional style alteration in mice

Objectives: It is well established that diet and lifestyle are important in maintenance of healthy. Transition from a plant-based diet mostly to a high-calorie diet of animal products might raise the chronic diseases which called “degenerative”. This work aimed to study the histopathological effect of transition from complete plant-based diet to 10% animal products (sheep’s brain) on various body organs of mice. Methods: Eight-week old Balb/c male mice were divided into 2 groups (n=8); the first is restricted group in which mice were fed on restricted diet containing 10% of sheep’s brain homogenate, while the second is the control group in which fed on ad libitum on the diet for 7 days. During the duration of experiment, body weight and the amount of food intake were recorded daily, then at the end of experiment, all mice were sacrificed and various organs were obtained and processed for histopathological study. Results: the results showed that food intake by each mouse of restricted group are significantly lower than in control group. Although the mean of body weight in both groups revealed non-significant difference, the relative weight of various organs showed significant differences. On the other hand, sever histological changes were detected in all studied organs sections of restricted group. Conclusion: It can be concluded that changing in nutritional style rather than conventional diet play a crucial role in modifying the architectural aspects of different organs at tissue level. Therefore, these findings need further investigation at cellular, physiological, and molecular levels.