Open AccessSynthesis, Characterization, in vivo acute toxicity and superoxide anion scavenging evaluation of new isatin-hydrazone
Author(s) -
Kamel Mokhnache,
Ahlem Karbab,
Soraya Madoui,
Hanane Khither,
El-Khamsa Soltani,
Noureddine Charef
Publication year - 2019
Publication title -
journal of drug delivery and therapeutics
Language(s) - English
Resource type - Journals
ISSN - 2250-1177
DOI - 10.22270/jddt.v9i6.3656
Subject(s) - hydrazone , chemistry , superoxide , acute toxicity , antioxidant , isatin , carbohydrazide , nuclear chemistry , toxicity , organic chemistry , enzyme
A new isatin-hydrazone (I); N'-[(E)-(5-bromo-1H- indol-3-yl) methylidene] pyridine-4-carbohydrazide was prepared from the condensation reaction of 5-bromo-1H-indole-3-carbaldehyde and the anti-tubercular drug; isoniazid, in the presence of acetic acid. The obtained hydrazone was identified and characterized by physico-chemical techniques such as melting point, IR, NMR, and mass spectroscopy. In addition, the acute toxicity was evaluated using mice. The antioxidant of I was evaluated against superoxide anion radical. Our biological results indicate low toxicity of I at the high dose of 1000 mg/kg, and high superoxide anion scavenging effect with inhibition percentage of 82.57 % and IC50 138.78 µg/mL.
Keyword: hydrazone, toxicity, antioxidant, superoxide anion