Open AccessUV-Spectrophotometric Estimation of Olopatadine hydrochloride in Bulk and Pharmaceutical Dosage Form by Zero, First and Second Order Derivative Methods
Author(s) -
Smita S. Aher,
Jayshree S. Gawali,
R.B. Saudagar
Publication year - 2019
Publication title -
journal of drug delivery and therapeutics
Language(s) - English
Resource type - Journals
ISSN - 2250-1177
DOI - 10.22270/jddt.v9i4-s.3378
Subject(s) - zero order , dosage form , derivative (finance) , mathematics , relative standard deviation , pharmaceutical formulation , second derivative , chromatography , spectrophotometry , chemistry , first order , detection limit , mathematical analysis , financial economics , economics
Simple and accurate UV spectrophotometric methods by Zero, First and Second order derivative method have been developed and validated for the estimation of Olopatadine hydrochloride in bulk and its pharmaceutical dosage form. The standard and sample solutions of Olopatadine hydrochloride were prepared in methanol and water. Olopatadine hydrochloride was estimated at 299, 289 and 267 nm for the derivative UV-spectrophotometric method. Beer’s law was obeyed in the concentration range of 20 to 120 μg / mL with coefficient of correlation value 0.9996, 0.999 and 0.999 for Zero, First and Second order derivative method. These methods were tested and validated for various parameters according to ICH and USP guidelines. The precision expressed as relative standard deviation were of less than 2 for the above three methods respectively. The proposed methods were successfully applied for the determination of Olopatadine hydrochloride in pharmaceutical dosage form. Results of the analysis were validated statistically and were found to be satisfactory. The proposed methods are simple, easy to apply, low-cost and require relatively inexpensive instruments.
Keywords: Olopatadine HCl, UV-Visible spectrophotometry, Pharmaceutical Dosage forms, Derivative Spectroscopy, Method validation.