Open AccessWound healing promoting activity of Earthworm, Eutyphoeus gammiei (Beddard): in vitro studies on human skin keratinocyte cell line (HaCat).
Author(s) -
Susmita Saha,
Deepjyoti Bhattacharjee,
Anwesha Saha,
Gahin De,
Priya Saha,
Samir Kumar Sil
Publication year - 2018
Publication title -
journal of drug delivery and therapeutics
Language(s) - English
Resource type - Journals
ISSN - 2250-1177
DOI - 10.22270/jddt.v8i6.2036
Subject(s) - hacat , keratinocyte , wound healing , cell culture , mtt assay , cell growth , in vitro , cell migration , chemistry , microbiology and biotechnology , biology , immunology , biochemistry , genetics
Earthworm, Eutyphoeus gammiei, homogenate (EGH) was screened for wound healing activity on human keratinocyte cell line, HaCat, by cell proliferation and migration assays. The maximum proliferation and migration of keratinocyte cells were observed at the dose of 25μg/ml. As cell proliferation and migration are key factors for wound healing, the study clearly suggests the potential role of earthworm species Eutyphoeus gammiei on wound healing.
Keywords: Eutyphoeus gammiei, Keratinocyte, MTT assay, scratch assay.