Extraction, Isolation and Standardization of Herbal Species Sida

The main focus of present is study is to find out the various extracts and active constituents from the Sida species which will be very useful in the treatment of many diseases. There are mainly four species (Sida acuta, Sida cardifilia, Sida rhombifolia and Sida spinosa) focused in this research. The aerial part and roots of these species have been collected and authenticated from botany department. The exhaustive solvent extractions have been used based on polarity. The aqueous solvents water, methyl alcohol, ethyl alchol and acetic acids have been used and non-aqueous solvents petroleum ether, ethyl acetate, chloroform, carbon tetra chloride, hexane, heptanes, benzene, toluene have been used for the extraction of active medicaments. The extracts were collected and purified and then tested for pharmacological activities. The extracts or residue are further processed for the isolation of active constituents like vascine, ephedrine etc. These were separated and quantified by high performance thin layer chromatography. Keywords: Sida acuta, Sida cardifolia, vasicine ephedrine, Extraction, Thin layer chromatography, High performance liquid chromatography.