Open AccessInhibition of α-amylase activity of Chamaecrista nigricans (Vahl) Greene (Fabaceae-Caesalpinioideae) and Pseudocedrela kotschyi (Schweinf.) Harms (Meliaceae) water extracts
Author(s) -
Yacouba Sanou,
Martin Bienvenu Somda,
Lassina Ouattara,
Flora Anne Claire Drabo,
Relwendé Justin Ouédraogo,
Roland Nâg-Tiéro Meda,
Georges Anicet Ouédraogo
Publication year - 2021
Publication title -
journal of drug delivery and therapeutics
Language(s) - English
Resource type - Journals
ISSN - 2250-1177
DOI - 10.22270/jddt.v11i6.5121
Subject(s) - abts , dpph , chemistry , traditional medicine , antioxidant , amylase , trolox , food science , enzyme , biochemistry , medicine
Antioxidants and glucosidases inhibitors are interesting in diabetes mellitus prevention and its management. However, conventional drugs have a multitude of unenviable side effects. Thus, research is turning towards herbal remedies, hence this study that was interested in two plants used in the traditional treatment of diabetes in Bobo-Dioulasso. Objective: The aim was to evaluate Chamaecrista (C.) nigricans and Pseudocedrela (P.) kotschyi roots water extracts effect on sorghum α-amylase activity.
Methodology: Total phenolic and flavonoid contents were quantified using Folin-Ciocalteu reagent and aluminum trichloride, respectively. FRAP, DPPH and ABTS methods were used to estimate the extracts antioxidant activity. The α-amylase inhibition tests were carried out using the glucose assay method with dinitrosalicylic acid.
Results: The C. nigricans and P. kotschyi extracts have shown strong antioxidant activities, 7031.52±15 and 8762.35±51 µmol AAE.g-1E respectively, referring to trolox using ABTS method. These extracts have also shown an inhibitory effect of 98.57% and 97.67% respectively on α-amylase activity.
Conclusion/perspectives: These plants have in vitro an interesting anti-amylasic potential. Further investigations will be necessary using a pure enzyme and a wider range of concentration of the extracts in order to determine the kinetic parameters and the fractionation by chromatography to determine the most effective fraction(s). Finally, an in vivo evaluation of the anti-diabetic effect of the extracts could be considered.
Keywords: anti α-amylase, antioxidant, Chamaecrista nigricans, Pseudocedrela kotschyi.