Date Powder as Alternative Carbone Source in Optimizing the Cultivation of (Bacillus megaterium) in Submerged State Fermentation

Problems statement: A study had shown a strain of Bacillus megaterium was isolated from the soil. Questions were whether date powder could be used as alternatives of carbon sources for Bacillus megaterium submerged state cultivation? Approach: To evaluate  the effects of the using date powder; The fermentation was carried out in shake flasks using a complex medium consisting of (g/L) ammonium tartrate, 2.0; magnesium sulphateheptahydrate, 4.0; dipotassium phosphate, 14.0; calcium chloride, 0.2, NaHPO4, 4.0, yeast extract, 3.0, trace elements, 2.0ml and glucose 6.0(4.0, yeast extract, 3.0, trace elements, 2.0ml and glucose 6.0) were replaced by dates powder at three rates; 5,10 and15 g/L Results show that the rate of production of biomass during submerged fermentation by Bacillus megaterium at 30±1°C for 24 hours of incubation using date powder was maximizes between 18 and 24 hr of fermentation. The experimental runs and results for the Box– Behnken design. The 15 runs in a single block were used to study the effects using date powder in different rates on one response. Biomass concentration ranged from 6.5cfu /l to 12.9cfu /.comparing with 14.8 cfu/ g for the commercial growth media. The ANOVA tables give the statistical significance of the effects for biomass. These effects were dates powder rates, the optimized growth medium with pH 7, inoculums’ size 106, temp 24oC and moisture dates powder 15 g/L. The biomass concentrations obtained under the optimal conditions 12.9cfu/l. Key words: Date Powders, Fermentation, Bacillus megaterium, biomass, Submerged State