Open AccessMethod Development and Validation of Polmacoxib in Capsule Dosage Form by RP-HPLC
Author(s) -
Anjali Chaudhary,
Meenakshi Dhaiya,
Shaily Tyagi,
Swati Mittal
Publication year - 2021
Publication title -
journal of drug delivery and therapeutics
Language(s) - English
Resource type - Journals
ISSN - 2250-1177
DOI - 10.22270/jddt.v11i4-s.4984
Subject(s) - chromatography , high performance liquid chromatography , gradient elution , linearity , capsule , elution , detector , computer science , chemistry , physics , telecommunications , botany , quantum mechanics , biology
A simple and sensitive RP-HPLC method developed for estimation of Polmacoxib in bulk by using a high performance liquid chromatography with PDA detector using Phenomenex luna C18 column (250mmx4.6mm), 5 µm; mobile phase comprises of Water : ACN as (1:1) at the flow rate 1.0 ml/min and the wavelength of detection 238 nm and eluted at 8.12 minutes. The proposed method is validated for specificity, Linearity, Precision, accuracy, ruggedness, and Robustness. All the parameters were found within the acceptable limits. RP-HPLC method was a simple, reliable economic and acceptable and it confirmed that method is suitable for the intended use for routine quality control and assay of drugs. This method is successfully applied for the determination of commercial dosage form capsule preparation. This method is validated as per ICH (International conference on harmonization) Guidelines.
Keywords: Polmacoxib, RP-HPLC, ICH, PDA Detector, Precision