Open AccessEvaluation of Antioxidant Activity and Polyphenols Content of the Hydro-methanolic Extract from Saccocalyx satureioides Coss and Dur
Author(s) -
Soulaf Mehlous,
Fatima Benchikh,
Hassiba Benabdallah,
Karima Loucif,
Chahrazed Kaoudoune,
Hocine Laouer,
Smain Amira
Publication year - 2020
Publication title -
journal of drug delivery and therapeutics
Language(s) - English
Resource type - Journals
ISSN - 2250-1177
DOI - 10.22270/jddt.v10i4.4166
Subject(s) - gallic acid , chemistry , dpph , polyphenol , flavonoid , antioxidant , quercetin , food science , traditional medicine , chromatography , organic chemistry , medicine
This study aimed to estimate the total phenolic and flavonoid contents and to evaluate the antioxidant activity of the Hydro-methanolic from Saccocalyx satureioides Coss and Dur aerial part. Total polyphenols contents were determined using Folin-Ciocalteu’s reagent. The flavonoids were estimated using the method of Aluminum chloride (AlCl3). The antioxidant capacity was evaluated using two in vitro models (the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and reducing power). Total phenolic and flavonoid content in the extract were 171.34 ±1.43 mg Gallic acid equivalent/g of dry extract (GAE/g) and 18.6 ± 0.46 mg Quercetin equivalent / g of dry extract (QE/g), respectively. The methanol extract has an important capacity to scavenge the free radical DPPH with an IC50 of 0.03 ± 0. 0024 mg/ml. In addition, the plant extract exhibited high reducing power with an IC50 of 0.54 ± 0.00625 mg/ml. The results of the present study may prove that the medicinal plants are a good resource of natural antioxidants.
Keywords: Antioxidant activity, Saccocalyx satureioides, polyphenols, flavonoids.