Open AccessPreparation and Evaluation of Sodium Alginate Microparticles using Pepsin
Author(s) -
Sankha Bhattacharya,
Vishal Puri,
Shubham Sharma,
Debasish Sahoo,
Pooja Pal,
Sima Kujur,
Gaurav Goyal,
Amandeep Singh
Publication year - 2020
Publication title -
journal of drug delivery and therapeutics
Language(s) - English
Resource type - Journals
ISSN - 2250-1177
DOI - 10.22270/jddt.v10i2.3901
Subject(s) - zeta potential , particle size , chromatography , chemistry , sodium alginate , calcium alginate , particle size distribution , sodium , pepsin , active ingredient , particle (ecology) , chemical engineering , calcium , materials science , nanotechnology , nanoparticle , biochemistry , organic chemistry , pharmacology , medicine , enzyme , oceanography , geology , engineering
Aim: The main aim of this article is to prepare and evaluate sodium alginate microparticles and evaluate on the basis of their characterization. The drug is dissolved, encapsulated or attached to a microparticles matrix. Depending upon method of preparation microparticles were obtained. Microparticles were developed as a carrier for vaccines and other disease like rheumatoid arthritis, cancer etc. Microparticles were developed to increase the efficacy of active pharmaceutical ingredient to a specific targeted site.
Material and Method: Microparticles of Sodium Alginate, Pepsin and Calcium Chloride were prepared in six batches (A-F) with different ratio of sodium alginate and calcium chloride respectively i.e. (0.25:2.5), (0.25:5), (0.25:7.5), (0.5:2.5), (0.5:5), (0.5:7.5) by using a homogenizing method. Microparticles were evaluated for particle size distribution, zeta potential and morphology.
Result and Discussion: The normal particle size of each of the six batches were analyzed by Zeta Sizer (Delsa C Particle Analyzer) and it was found that the Batch B (0.25:5) delivered the best microparticles with size distribution of 1.2731 (µm). All batches were seen under Motic magnifying microscope by using the Sulforhodamine B (M.W. 479.02) color as staining dye. Microparticles was found to be semi spherical in shape.
Conclusion: Results of all the six batches was contrasted based on particle size investigation, zeta potential and morphology. Batch B (0.25:5) was considered as the best formulation.
Key words: Micro Particle, Pepsin, Sodium Alginate and Calcium Chloride, Sulforhodamine B, Zeta Sizer.