Open AccessApplication of Red Dragon Fruit Dyes as Staining in Histological Study
Author(s) -
Ridho Asra,
R Rusdi,
Indah Kemala Fitrina,
Nessa Nessa
Publication year - 2021
Publication title -
asian journal of pharmaceutical research and development
Language(s) - English
Resource type - Journals
ISSN - 2320-4850
DOI - 10.22270/ajprd.v9i6.1045
Subject(s) - h&e stain , flesh , eosin , histology , staining , pigment , eosin y , melanin , neutral red , biology , chemistry , horticulture , biochemistry , organic chemistry , photocatalysis , cytotoxicity , in vitro , catalysis , genetics
Background: The betacyanin pigment found in dragon fruit flesh (Hylocereus costaricensis (F.A.C.Weber) Britton & Rose) has many advantages, for instance as a tissue histology dye.
Objectives: determining the properties of organs stained with betacyanin red dye and finding alternative histology colors to replace synthetic hematoxylin-eosin dyes.
Summary of contents of the article: The flesh of the red dragon fruit (Hylocereus costaricensis (F.A.C.Weber) Britton & Rose) was extracted using the Ultrasonic Assisted Extraction (UAE) method. The study employed six male white mice divided into two treatment groups: a placebo group induced with 1% Na.CMC carrier solution and a positive control group generated a toxic dose of paracetamol. The results were visually compared to hematoxylin-eosin with standard betacyanin and betacyanin extract of red dragon fruit flesh concentrations of 50% (v/v), 80% (v/v), and 100% (v/v).
Conclusion: Betacyanin from the flesh of the red dragon fruit can be applied as a histological dye in the liver of male white mice and as a counterstaining hematoxylin as a substitute for eosin dye at a concentration of 100% (v/v).