Open AccessInhibitory AKT2 And Vegr2 Genes Expression of Avicennia Marina Using RT-PCR On Widr Cells
Author(s) -
Taufiq Qurrohman,
Poppy Anjelisa Zaitun Hasibuan,
Mohammad Basyuni
Publication year - 2020
Publication title -
asian journal of pharmaceutical research and development
Language(s) - English
Resource type - Journals
ISSN - 2320-4850
DOI - 10.22270/ajprd.v8i1.641
Subject(s) - avicennia marina , protein kinase b , reverse transcription polymerase chain reaction , biology , hexane , microbiology and biotechnology , gene expression , gene , chemistry , biochemistry , signal transduction , mangrove , fishery , chromatography
Objectives: To evaluate the activities the anticancer of n-hexane extract of Avicennia marina leaves on WiDr cells in down-regulated of expression of Akt2 and VEGFR2 genes.
Methods: Avicennia marina leaves were dried and extracted with n-hexane, analyzed the down-regulated Akt 2 and VEGFR2 expression which was determined the Reverse Transcription Polymerase Chain Reaction (RT-PCR) method.
Results:N-hexane extract of Avicennia marina, in which there were down regulated expression Akt 2 and VEGFR2 of 0.43 and 0.50 WiDr cells. N-hexane extract of mangrove leaves has cancer chemoprevention activity with down-regulated on WiDr cells.
Conclusions: :N-hexane extract of Avicennia marina leaves had anticancer activity with down-regulated on WiDr cells, suggest that significantly inhibit the expression of Akt2 and VEGFR2 genes.