Open AccessAnalysis of Vannamei shrimp DNA fragment resistant to White Spot Virus Syndrome
Author(s) -
Wiwien Mukti Andriyani,
Sumini Sumini,
Zeny Widiastuti
Publication year - 2021
Publication title -
indonesian journal of tropical aquatic
Language(s) - English
Resource type - Journals
eISSN - 2721-1657
pISSN - 2622-4836
DOI - 10.22219/ijota.v4i1.15839
Subject(s) - shrimp , white spot syndrome , rapd , biology , dna , polymerase chain reaction , virus , penaeidae , selection (genetic algorithm) , virology , gene , genetics , microbiology and biotechnology , decapoda , fishery , crustacean , genetic diversity , population , medicine , environmental health , artificial intelligence , computer science
The attack of WSSV in Vannamei shrimp cultivation is still common. Shrimp quality improvement can be made through selection with the help of markers (marker-assisted choice). This study aimed to evaluate the DNA fragment profile of white shrimp that was resistant to WSSV disease. The analysis was performed using the PCR-RAPD method. WSSV challenged four groups of 100 Vannamei shrimp, then DNA was extracted from live and dead shrimp. The results showed that 2 of the 17 primers tested had high potential as markers, namely OP-09 and OPD-2. PCR products with OPC-09 primers had specific DNA bands measuring about 1.2 kb in all post-challenge WSSV resistant shrimp individuals. The amplification results using OPD-02 primers showed a particular band of DNA with a length of about 1.0 kb, with 60 % of the appearance in WSSV-resistant shrimp. In contrast, the WSSV-susceptible shrimp group did not have specific DNA fragments. Thus, the two RAPD primers had a high chance of being used in the selection with the help of markers to produce WSSV resistant shrimp.