Open AccessStudy of Physiological Parameters and Rheology for the Production and Extraction of Cyclosporin A
Author(s) -
P. Jawahar Babu,
Yogesh C. Patel,
Subhashis Pany,
Ilma Majeed,
Umesh Luthra
Publication year - 2022
Publication title -
international journal for research in applied science and engineering technology
Language(s) - English
Resource type - Journals
ISSN - 2321-9653
DOI - 10.22214/ijraset.2022.41268
Subject(s) - fermentation , aeration , extraction (chemistry) , rheology , food science , sugar , downstream processing , productivity , chemistry , chromatography , microbiology and biotechnology , biology , materials science , organic chemistry , economics , composite material , macroeconomics
This study is an evaluative effort to analyse different physiological parameters and rheological properties observed during the fermentative production of cyclosporin A. Tolypocladium inflatum culture was used for seed development. For production fermentation, the mature seed was inoculated at specific transfer parameters. At each stage of the current study, pH, PMV and sugar consumption were keenly analysed and recorded. Different aeration rates and agitation speeds were evaluated during various stages of growth. Based on several experiments, the most optimised parameters were included for further analysis. The maximum productivity obtained was 8.3 g/L. Further, different kinds of solvents were studied to extract crude from the fermentation broth. Solvents required at various stages of downstream processing were analysed for best recovery with minimal product loss. Finally, the product was obtained with 80.58% recovery. Keywords: cyclosporin A, Tolypocladium inflatum, fermentation, seed, pH, PMV, extraction, downstream processing