In Vivo Protein Profiling & Estimation of Plant Secondary Metabolites, Phylogenetic Analysis of Plant Leaves for Antifungal Activity

The present research work involved to evaluated the phytochemical screening on different plant leaf extracts, protein profiling and phylogenetic analysis of leaf extracts which is Psidium guajava, Syzygium cumini, Aegle marmelos, Nyctanthes arbor-tristis, Callistemon lanceolatus, Citrus limonum, Combretum indicum, Calliandra haematocephala, Polyalthia longifolia were used fresh leaves of the plants and analysed the phylogenetic analysis between all plant leaf which having antifungal activity against plant pathogens .According to study it was found that several plant leaf contain different bands of different molecular weight which is Nyctanthes arbortristis 80KDa, 72KDa ,70KDa, 63KDa, 61KDa, 53KDa, 50KDa, 41KDa, 38KDa, 37KDa. Aegle marmelos 72KDa, 70KDa, 64KDa, 54KDa, 54KDa, 40KDa, 36KDa, 35KDa. Polyalthia longifolia 75KDa, 70KDa, 44KDa, 41KDa, 35KDa. Psidium guajava 75KDa, 70KDa, 54KDa, 53KDa, 45KDa, 40KDa, 35KDa.The protein profiling of the different leaf extracts Psidium guajava, Syzygium cumini, Aegle marmelos, Nyctanthes arbor-tristis, Callistemon lanceolatus, Citrus limonum, Combretum indicum, and Calliandra haematocephala, Polyalthia longifolia gave an idea that proteins and peptides present in the leaves. The proteins also may act as antifungal agents. Thus, after the protein profiling was done, based on this the UPGMA (Un-weighted paired group method with arithmetic average) software was used to identify the evolutionary presence of plant. The evolutionary relationship has thus been depicted between the different trees.