Open AccessIn vitro Antibiosis of Chlorella vulgaris Extract against the Phytopathogen, Stenotrophomonas maltophilia
Author(s) -
Mohammed A. Almalki,
Ashraf Khalifa,
Yousef Ahmed Alkhamis
Publication year - 2022
Publication title -
journal of pure and applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.149
H-Index - 16
eISSN - 2581-690X
pISSN - 0973-7510
DOI - 10.22207/jpam.16.1.64
Subject(s) - stenotrophomonas maltophilia , chlorella vulgaris , antibiosis , antimicrobial , agar diffusion test , biology , chemistry , stenotrophomonas , food science , antibacterial activity , traditional medicine , microbiology and biotechnology , bacteria , 16s ribosomal rna , botany , pseudomonas aeruginosa , algae , medicine , genetics
The aim of the current study was to evaluate the antibacterial activity of Chlorella vulgaris extracts against Stenotrophomonas maltophilia CSK1 isolated from a diseased cucumber fruit. To this end, water, methanol and ethanol were used separately as solvents to extract the active compounds from the dried Chlorella vulgaris. S. maltophilia CSK1 was identified using the 16S rRNA gene sequencing at 99.93% of identity level. The identity of the bacterial strain CSK1 was confirmed by phylogenetic analyses. CSK1 was clearly clustered with the reference strain, S. maltophilia MTCC 434 (JALV01000036). The antibiosis of the extract against S. maltophilia CSK1 was estimated using agar well diffusion method. Unlike water and ethanolic extracts, the methanolic one exhibited an inhibition of CSK1 (zone of inhibition 13.4 mm), indicating the antimicrobial activity of this extract. No zone of inhibition was formed around ethanolic and water extracts, indicating that both exhibited no-observed effect against the growth of CSK1. This can be attributed to the active constituents in the extract which may varies based on various factors including the polarity of solvent used in the extraction process. The inhibitory effect of the methanolic extract against the S. maltophilia CSK1 obtained from the diseased cucumber fruit could open the door for potential application of such active ingredient within this extract against this bacteria in future.