Open AccessOptimization of Keratinase Production and Utilization of Bacillus pumilus for Feather Degradation
Author(s) -
S. Dhiva,
C. Akshara,
K. Afna,
U. Dhanush,
Preeti Arya,
S. Saigeetha,
S. Abirami,
Angel Abraham,
R. Emilin Renitta,
Antony V. Samrot
Publication year - 2020
Publication title -
journal of pure and applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.149
H-Index - 16
eISSN - 2581-690X
pISSN - 0973-7510
DOI - 10.22207/jpam.14.4.26
Subject(s) - keratinase , bacillus pumilus , incubation , degradation (telecommunications) , feather , microbiology and biotechnology , incubation period , chemistry , food science , bacillus (shape) , enzyme , biology , bacteria , biochemistry , ecology , telecommunications , computer science , genetics
Soil samples were collected from the feather dumped area where Bacillus pumilus was isolated and used for keratinase production and keratinolytic activity. In the optimization study, optimal condition for enzyme production was observed at 144 h, pH 7, temperature 37°C. The organism was utilized for feather degradation study. The maximum degradation of 57% was obtained at 37°C, pH 7 and 6 days incubation. The size of keratinase was determined by SDS- PAGE and was observed as 52 KDa.