Open AccessAdipose-derived stem cell exosomes facilitate rotator cuff repair by mediating tendon-derived stem cells
Author(s) -
Guojian Fu,
Liangyu Lu,
Zhangyi Pan,
Aoyuan Fan,
Feng Yin
Publication year - 2021
Publication title -
regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 63
eISSN - 1746-076X
pISSN - 1746-0751
DOI - 10.2217/rme-2021-0004
Subject(s) - rotator cuff , stem cell , mesenchymal stem cell , microvesicles , adipose tissue , tendon , runx2 , microbiology and biotechnology , medicine , chemistry , in vitro , pathology , surgery , biology , microrna , osteoblast , biochemistry , gene
Aim: To evaluate the potential capability of adipose-derived stem cell exosomes (ADSC-exos) on rotator cuff repair by mediating the tendon-derived stem cells (TDSCs) and explored the mechanism. Methods: First, we investigated the growth, survival and migration of TDSCs in the presence of ADSC-exos in vitro. Using a rat rotator cuff injury model to analyze the ability of the ADSC-exos to promote rotator cuff healing in vivo. Results: The hydrogel with ADSC-exos significantly improved the osteogenic and adipogenesis differentiation and enhanced the expression of RUNX2, Sox-9, TNMD, TNC and Scx and the mechanical properties of the articular portion. Conclusion: The ADSC-exos have the potential to promote the rotator cuff repair by mediating the TDSCs.