z-logo
open-access-imgOpen Access
Conditioned medium from 3D culture system of stromal vascular fraction cells accelerates wound healing in diabetic rats
Author(s) -
Chao Deng,
Yunfan He,
Jiaxuan Feng,
Ziqing Dong,
Yao Yao,
Feng Lu
Publication year - 2019
Publication title -
regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 63
eISSN - 1746-076X
pISSN - 1746-0751
DOI - 10.2217/rme-2018-0083
Subject(s) - wound healing , stromal cell , stromal vascular fraction , medicine , diabetes mellitus , pathology , endocrinology , surgery
Aim: We investigated the healing effects of conditioned medium (CM) derived from a physiological 3D culture system engineered to use an extracellular matrix/stromal vascular fraction (SVF) gel enriched for adipose on diabetic wounds in rats. This CM (Gel-CM) was compared with that from a 2D culture system that used SVF cells (SVF-CM). Materials & methods: Keratinocytes, fibroblasts and wounds were treated with Gel-CM and SVF-CM, and cytokine levels in the CM types were quantified. Results: Proliferation and migration of keratinocytes and fibroblasts were significantly higher after treatment with Gel-CM than with SVF-CM. Collagen secretion by fibroblasts and wound closure were highly stimulated by Gel-CM. Proteomic analyses revealed a higher concentration of growth factors in Gel-CM than in SVF-CM. Conclusion: Gel-CM is a promising therapeutic option for treating diabetic wounds.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.
Having issues? You can contact us here