Open AccessPrognostic value of intratumoral lymphocyte-to-monocyte ratio and M0 macrophage enrichment in tumor immune microenvironment of melanoma
Author(s) -
Neil Jairath,
Mark Farha,
Ruple Jairath,
Paul W. Harms,
Lam C. Tsoi,
Trilokraj Tejasvi
Publication year - 2020
Publication title -
melanoma management
Language(s) - English
Resource type - Journals
eISSN - 2045-0893
pISSN - 2045-0885
DOI - 10.2217/mmt-2020-0019
Subject(s) - melanoma , immune system , monocyte , tumor microenvironment , phenotype , biology , immune checkpoint , lymphocyte , immunotherapy , macrophage , immunology , transcriptome , proportional hazards model , cancer research , medicine , gene , gene expression , genetics , in vitro
Skin cutaneous melanoma is characterized by significant heterogeneity in its molecular, genomic and immunologic features. Whole transcriptome RNA sequencing data from The Cancer Genome Atlas of skin cutaneous melanoma (n = 328) was utilized. CIBERSORT was used to identify immune cell type composition, on which unsupervised hierarchical clustering was performed. Analysis of overall survival was performed using Kaplan–Meier estimates and multivariate Cox regression analyses. Membership in the lymphocyte:monocyte low , monocyte hi gh and M0 high cluster was an independently poor prognostic factor for survival (HR: 3.03; 95% CI: 1.12–8.20; p = 0.029) and correlated with decreased predicted response to immune checkpoint blockade. In conclusion, an M0-macrophage-enriched, lymphocyte-to-monocyte-ratio-low phenotype in the primary melanoma tumor site independently characterizes an aggressive phenotype that may differentially respond to treatment.