Open AccessDendritic polyglycerols are modulators of microglia-astrocyte crosstalk
Author(s) -
Dušica Maysinger,
Mélanie Lalancette–Hébert,
Jeff Ji,
Katherine Jabbour,
Jens Dernedde,
Kim Silberreis,
Rainer Haag,
Jasna Križ
Publication year - 2019
Publication title -
future neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.419
H-Index - 32
eISSN - 1748-6971
pISSN - 1479-6708
DOI - 10.2217/fnl-2019-0008
Subject(s) - microglia , astrocyte , crosstalk , microbiology and biotechnology , in vivo , chemistry , tlr2 , stimulation , lipopolysaccharide , signal transduction , tlr4 , inflammation , biology , neuroscience , immunology , central nervous system , physics , optics
Aim: To determine the ability of sulfated dendritic polyglycerols (dPGS) to modulate neuroglia activation challenged with lipopolysaccharide (LPS). Materials & methods: Microglia/astrocyte activation in vivo was determined in transgenic animals expressing TLR2-/GFAP-luciferase reporter. Mechanisms implicated in microglia-astrocyte crosstalk were studied in primary mouse brain cultures. Results & discussion: dPGS significantly reduced microglia activation in vivo, and decreased astrocytic LCN2 production. Activated microglia are necessary for astrocyte stimulation and increase in LCN2 abundance. LCN2 production in astrocytes involves signaling via toll-like receptor 4, activation of NF-κB, IL6 and enhancement of reactive oxygen species. Conclusion: dPGS are powerful modulators of microglia-astrocyte crosstalk and LCN2 abundance; dPGS are promising anti-inflammatory dendritic nanostructures.