Open AccessSynergistic action of substituted indole derivatives and clinically used antibiotics against drug-resistant bacteria
Author(s) -
Danielle N. Turner,
Leslie Edwards,
Alexander Kornienko,
Liliya V. Frolova,
Snezna Rogelj
Publication year - 2020
Publication title -
future microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.797
H-Index - 82
eISSN - 1746-0921
pISSN - 1746-0913
DOI - 10.2217/fmb-2019-0094
Subject(s) - antibiotics , indole test , staphylococcus aureus , microbiology and biotechnology , bacteria , drug , viability assay , bacterial cell structure , vancomycin , antibiotic resistance , multiple drug resistance , drug resistance , chemistry , biology , pharmacology , in vitro , biochemistry , genetics
Aim: The current report describes the discovery of indole derivatives that synergize with standard antibiotics. Materials & methods: The antibacterial activities were determined using an optimized time-kill method, while viability of mammalian cells was assessed using the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Results: The synergy is observed with methicillin- and vancomycin-resistant Staphylococcus aureus bacterial strains, against which the standard antibiotics show no activities of their own. Our indole derivatives in combination with antibiotics lack toxicity toward mammalian cells, do not promote the evolution of resistance of S. aureus in comparison to clinically established antibiotics, and likely work by permeabilizing bacterial cell membranes. Conclusion: The above-mentioned findings demonstrate the potential clinical applications of our indole derivatives.