Open AccessIN VITRO ANTI-INFLAMMATORY ACTIVITY OF SYRINGIC ACID
Author(s) -
Shilpee Chanda,
Archana R. Juvekar
Publication year - 2018
Publication title -
international journal of pharmacy and pharmaceutical sciences/international journal of pharmacy and pharmaceutical sciences
Language(s) - English
Resource type - Journals
eISSN - 2656-0097
pISSN - 0975-1491
DOI - 10.22159/ijpps.2019v11i2.30387
Subject(s) - haemolysis , syringic acid , chemistry , ic50 , hemolysis , in vitro , denaturation (fissile materials) , diclofenac sodium , biochemistry , pharmacology , microbiology and biotechnology , chromatography , biology , immunology , nuclear chemistry , antioxidant , gallic acid
Objective: The present study was carried out to investigate the in vitro anti-inflammatory activity of syringic acid (SA).
Methods: SA was tested for it's in vitro anti-inflammatory activity at different concentrations in protein denaturation, proteinase inhibition and human red blood cell (HRBC) membrane stabilization assay. The reference drugs used were aspirin and diclofenac sodium.
Results: SA showed concentration-dependent inhibition of protein denaturation and proteinase activity with a half-maximal inhibitory concentration (IC50) value of 49.38±0.56 µg/ml and 53.73±0.27 µg/ml respectively. Heat-induced haemolysis was inhibited by SA with an IC50 value of 57.13±0.24 µg/ml. SA also inhibited the hypotonicity-induced haemolysis (IC50 value of 53.87±0.72 µg/ml).
Conclusion: From the present study, we can conclude that SA possesses appreciable anti-inflammatory effect against denaturation of proteins, proteinase activity, and human red blood membrane stabilization assays. Further studies are required for determining the possible mechanisms behind its anti-inflammatory action.