Open AccessIMMUNOMODULATORY, ANTICANCEROUS & ANTIOXIDANTACTIVITIES OF CYCLEA PELTATA (LAM.) HOOK.F. & THOMSON
Author(s) -
Sony Jayaraman,
E. Jayadevi Variyar
Publication year - 2019
Publication title -
international journal of pharmacy and pharmaceutical sciences/international journal of pharmacy and pharmaceutical sciences
Language(s) - English
Resource type - Journals
eISSN - 2656-0097
pISSN - 0975-1491
DOI - 10.22159/ijpps.2019v11i10.35248
Subject(s) - antioxidant , dpph , chemistry , flavonoid , traditional medicine , ascorbic acid , mtt assay , pharmacology , biochemistry , biology , in vitro , food science , medicine
Cyclea peltata (Lam.) Hook.f. & Thomson is an important plant listed in Ayurveda. The plants are used in digestive, diuretic and inflammatory disorders.
Aim: The present study was to study the immunomodulatory, anticancerous and antioxidative properties of the fraction isolated from Cyclea peltata.
Methods: The immunomodulatory activity was evaluated in lymphocytes and THP-1 macrophage cell lines. The anticancer activity of the fraction was determined by MTT assay. The antioxidant activity was evaluated by DPPH and total antioxidant assays.
Results: The fraction showed the presence of flavanoids which induced lymphocyte proliferation and also could induce nitrite production in THP-1 cell lines at 1mg/ml. It also exhibited good anticancerous activity at 100 μg/ml after 48h of incubation. The antioxidant activity was less at this concentration and was found to be equivalent to 79± 0.03 μg/ml of ascorbic acid.
Conclusion: The fraction containing flavonoid, isolated from Cyclea peltata have shown to have good immunomodulatory, antioxidant and anticancerous property.