Open AccessEVALUATION OF HEAMAGGLUTINATION AND ANTI-CANCER POTENTIAL FROM INDIAN DIETARY PLANTS
Author(s) -
Cherukury Madhu,
A. C. Sharada
Publication year - 2018
Publication title -
international journal of pharmacy and pharmaceutical sciences/international journal of pharmacy and pharmaceutical sciences
Language(s) - English
Resource type - Journals
eISSN - 2656-0097
pISSN - 0975-1491
DOI - 10.22159/ijpps.2018v10i7.27110
Subject(s) - ehrlich ascites carcinoma , lectin , cucumis , trypan blue , cucurbitaceae , in vitro , agglutination (biology) , hemagglutination , chemistry , biology , traditional medicine , biochemistry , pharmacology , botany , medicine , immunology , virus , antigen
Objective: To evaluate the agglutination and anti-cancer activity of different dietary plants, commonly used in Indian dietary system for its therapeutic applications.Methods: Sap/phloem exudates from different dietary plants were collected in phosphate buffer saline (PBS) was evaluated for lectin activity by using haemagglutination method in serial dilution manner. Anticancer activity of the different samples was evaluated against Ehrlich ascites carcinoma (EAC) cells by using trypan blue exclusion method.Results: Samples belongs to Cucurbitaceae family showed promising lectin activity among test samples. Praecitrullus fistulosus and Cucumis prophetarum give 256HU/mg whereas Cucumis dipsauses gives 128HU/mg activity, whereas Abelmoschus esculentus (16HU) which gives low lectin activity. Praecitrullus fistulosus shows anticancer activity 67.38% inhibition at 200µg concentration followed by Cucumis prophetum of 56.36% at 200µg concentration against Ehrlich ascites carcinoma (EAC) cells.Conclusion: The present study demonstrated that dietary lectin having a potential role in the biological field. High lectin activity demonstrates the anticancer effect in Ehrlich ascites carcinoma (EAC) model system, in vitro. Further study needs to isolate and evaluate biologically active lectin molecule in order to demonstrate the anticancer effect.