Open AccessGAS CHROMATOGRAPHIC DETERMINATION OF 23 ORGANOPHOSPHORUS PESTICIDES RESIDUE IN BOTTLE GUARD MATRIX
Author(s) -
Sanjaya Mishra,
Lalitesh K. Thakur,
Neelam Richhariya
Publication year - 2018
Publication title -
international journal of pharmacy and pharmaceutical sciences/international journal of pharmacy and pharmaceutical sciences
Language(s) - English
Resource type - Journals
eISSN - 2656-0097
pISSN - 0975-1491
DOI - 10.22159/ijpps.2018v10i1.21058
Subject(s) - quechers , chromatography , detection limit , pesticide , chemistry , phorate , residue (chemistry) , pesticide residue , gas chromatography , bottle , gas chromatography–mass spectrometry , mass spectrometry , materials science , biochemistry , agronomy , composite material , biology
Objective: To validate in-house QuEChERS method, which scrutinizes and quantify the residue levels of some most frequently used organophosphorus pesticides and to prove a complete workflow for routine multi-residue pesticide analysis in representative bottle guard matrices and study the persistence of 23 organ phosphorus pesticides.Methods: QuEChERS (quick, easy, cheap, effective, rugged and safe) extraction method was followed. Method validation and residue screening of bottle guard samples were conducted by GC-FPD (Gas Chromatography-Flame Photometric Detector) were used to analyse the presence of pesticides whereas confirmation of pesticides was done by GC-MS (Gas Chromatographic-Mass Spectrometer). Results: For bottle guard matrix, LOD (limit of detection) and LOQ (limit of quantification) values are lowest for phorate (0.005 mg/kg and 0.015 mg/kg) and highest for parathion-methyl (0.1 mg/kg and 0.3 mg/kg) respectively. Calibration curve, was plotted between an area of 23 pesticide standard mixture against seven different concentration levels of 0.01, 0.02, 0.05, 0.10, 0.20, 0.50, 1.00 mg/kg with regression coefficient (R2) are in the range of 0.978-0.994. The mean recovered amount at 0.05 mg/kg spikeing concentration are in the range of lowest 0.0413 mg/kg to highest 0.0467 mg/kg and relative standard deviation are in the range of lowest 2.2 to highest 8.571.Conclusion: For ensuring exposure to contaminants, especially by dietary intake, Robust analytical methods were validated for carrying out both research and monitoring programmes and thus for defining limitations and supporting enforcement of regulations.