Open AccessANTIMICROBIAL ACTIVITY AND TLC PROFILING OF CLERODENDRUM PHLOMIDIS LINN. F LEAF EXTRACT AGAINST MULTI-DRUG RESISTANT CLINICAL PATHOGENS
Author(s) -
A. Hannah Hepsibah,
M. Mala,
G. Jothi
Publication year - 2017
Publication title -
international journal of pharmacy and pharmaceutical sciences/international journal of pharmacy and pharmaceutical sciences
Language(s) - English
Resource type - Journals
eISSN - 2656-0097
pISSN - 0975-1491
DOI - 10.22159/ijpps.2017v9i9.20186
Subject(s) - petroleum ether , ethyl acetate , antimicrobial , chemistry , chromatography , thin layer chromatography , antibacterial activity , acetone , agar diffusion test , traditional medicine , bacteria , microbiology and biotechnology , biology , extraction (chemistry) , organic chemistry , medicine , genetics
Objective: The aim of the present study is to evaluate the antibacterial and antifungal activities of different solvent extracts of leaves of Clerodendrum phlomidis against multi-drug resistant clinical pathogens and to perform Thin Layer Chromatography (TLC) profiling of the extracts.Methods: The leaf powder was sequentially extracted with petroleum ether, ethyl acetate, acetone, and methanol. The extracts were tested for their antimicrobial activity against 20 bacterial strains and 15 fungal strains which were multiple drugs resistant by disc diffusion method. TLC profiling of all four extracts was performed.Results: The petroleum ether and ethyl acetate extracts showed antimicrobial activity against four bacteria and seven fungal strains. The highest zone of inhibition (18±0.50 mm) for bacteria was recorded against Staphylococcus epidermis by ethyl acetate extract and for fungi, the petroleum ether extract showed 13±0.00 mm against Trichophyton rubrum. TLC profiles showed that ethyl acetate extract possessed more compounds.Conclusion: From the results of the present study it is concluded that the petroleum ether and ethyl acetate extracts of C. phlomidis leaves are good sources of antimicrobial compounds which can inhibit multiple drug resistant pathogens.