Open AccessGANODERMA LUCIDUM-ORIENTAL MUSHROOM MEDIATED SYNTHESIS OF GOLD NANOPARTICLES CONJUGATED WITH DOXORUBICIN AND EVALUATION OF ITS ANTICANCER POTENTIAL ON HUMAN BREAST CANCER MCF-7/DOX CELLS
Author(s) -
D. S. Ranjith Santhosh Kumar,
P. Senthilkumar,
L. Surendran,
B. Sudhagar
Publication year - 2017
Publication title -
international journal of pharmacy and pharmaceutical sciences/international journal of pharmacy and pharmaceutical sciences
Language(s) - English
Resource type - Journals
eISSN - 2656-0097
pISSN - 0975-1491
DOI - 10.22159/ijpps.2017v9i9.20093
Subject(s) - colloidal gold , doxorubicin , conjugated system , mcf 7 , mtt assay , surface plasmon resonance , nuclear chemistry , chemistry , nanoparticle , cancer cell , materials science , apoptosis , nanotechnology , cancer , biochemistry , biology , organic chemistry , human breast , chemotherapy , genetics , polymer
Objective: The present investigations are to mycosynthesis and characterization of gold nanoparticles conjugated with doxorubicin and evaluated anticancer activity.Methods: The characterization of the gold nanoparticles using ultraviolet–visible spectroscopy. FTIR investigations were carried out to find and read the functional group responsible designed at the bioconversion of gold ions and crystalline arrangement of gold nanoparticles was detected in the XRD study. The gold nanoparticles conjugated with doxorubicin were treated against MCF-7-dox resisted breast cancer cells and observed the in vitro cytotoxicity by MTT assay, SCGE (Comet), Apoptosis and Mito-potential assay. Further more we determinate the mRNA expression of ABCB1 gene and cDNA was synthesized from the mRNA for amplification of the ABCB1 gene corresponding to the specific primer.Results: Surface Plasmon resonance showed the development of gold nanoparticles in UV–Visible spectra at 537 nm. The synthesized gold nanoparticles were polydisperse spherical and it was determined by EDAX and stabilized in the solution to the spherical shapes further confirmed by High-resolution transmission electron microscope analysis designate in the reading of 2–100 nm. The anticancer assays were given significant results and the mRNA expression of ABCB1 gene and cDNA was amplified as directly proportional to the expression of ABCB1 gene.Conclusion: We propose that gold nanoparticles synthesized and conjugated with doxorubicin from G. lucidum might be a significant resource of drug delivery for anti-cancer preparation that may advantage breast cancer treatment.