Open AccessPHYTOCHEMICAL SCREENING, ELEMENTAL AND FUNCTIONAL GROUP ANALYSIS OF VITEX NEGUNDO L. LEAVES
Author(s) -
Shubhangi M. Pawar,
Vanita Mohan Kamble
Publication year - 2017
Publication title -
international journal of pharmacy and pharmaceutical sciences/international journal of pharmacy and pharmaceutical sciences
Language(s) - English
Resource type - Journals
eISSN - 2656-0097
pISSN - 0975-1491
DOI - 10.22159/ijpps.2017v9i6.18093
Subject(s) - phytochemical , chemistry , anthraquinone , aqueous solution , elemental analysis , fourier transform infrared spectroscopy , vitex negundo , glycoside , nuclear chemistry , acetone , chloroform , chromatography , organic chemistry , biochemistry , physics , quantum mechanics
Objective: To analyse elemental profile, evaluate functional groups and find out phytochemical constituents of Vitex negundo L. leaves.Methods: Determination of primary and secondary metabolites for various extracts by qualitative methods. Elemental analysis was carried out by using inductively coupled plasma mass spectrometry (ICP-MS) technique and the functional groups have been determined by using Fourier Transform Infrared Spectroscopy (FTIR) technique.Results: Soluble extractive percentage of material has been found the maximum in the aqueous extract (6.75%) followed by methanolic extract (4.35%) and acetone extract (1.8%). Phytochemical screening of material revealed the presence of carbohydrates, proteins, amino acids, steroids, cardiac and anthraquinone glycosides, saponins, flavonoids, tannins and phenolic compounds. The elemental analysis revealed Na, Mg, K, Ca, Cr, Mn, Fe, Co, Cu, Zn, Se, Mo, Li, B, Al, P, Cd, As, Ba, and Hg. FTIR technique was used to identify various functional groups present in three different extracts of the material.Conclusion: The phytochemical screening of three different extracts of the material showed the presence of most of the primary and secondary metabolites in aqueous and methanolic extract than acetone extract. The soluble extractive value was found the maximum in aqueous extract hence aqueous extract is most effective for studying the pharmacological activity of this plant. Elemental analysis showed the presence of trace elements in sufficient concentrations and traces of heavy and toxic metals. The FTIR study revealed the presence of essential functional groups in three different extracts of the material. The present investigation is most essential to discover innovative, dynamic and novel drugs for curing various newly emerged dangerous health problems.