Open AccessANTIOXIDANT EFFECT OF POLYHERBAL FORMULATION IN ISOPRENALINE HYDROCHLORIDE INDUCED MYOCARDIAL INFARCTION IN RATS
Author(s) -
P. Kavitha,
C Sowmia
Publication year - 2017
Publication title -
international journal of pharmacy and pharmaceutical sciences/international journal of pharmacy and pharmaceutical sciences
Language(s) - English
Resource type - Journals
eISSN - 2656-0097
pISSN - 0975-1491
DOI - 10.22159/ijpps.2017v9i10.20310
Subject(s) - antioxidant , lipid peroxidation , isoprenaline , glutathione peroxidase , chemistry , pharmacology , oxidative stress , superoxide dismutase , catalase , hydrochloride , myocardial infarction , enzyme , biochemistry , medicine , stimulation
Objective: Myocardial infarction (MI) continues to be a major public health problem in the world. The prepared polyherbal formulation (PHF) exhibit cardioprotective effects by several mechanisms. The objective of the research was to evaluate the antioxidant effect of a polyherbal formulation in isoprenaline hydrochloride (ISO) induced myocardial infarction in rats.Methods: Oxidative stress and myocardial infarction was induced by administration of isoprenaline hydrochloride (25 mg/kg) for 2 d. The degree of protection was determined by measuring the levels of lipid peroxidation (LPO), enzymatic antioxidants (superoxide dismutase-SOD, Catalase, Glutathione peroxidase) and non-enzymatic antioxidants (Vitamin C, E and reduced glutathione-GSH).Results: Two different doses (250 mg and 500 mg/kg body wt) of the formulation was used to prove the cardioprotective effect. Pretreatment with polyherbal formulation significantly (P<0.05) lowered LPO and elevated the enzymatic and non-enzymatic antioxidants in heart homogenate.Conclusion: The polyherbal formulation exhibited cardioprotective activity, which could be attributed to its antioxidant activity.