Open AccessHYPOGLYCEMIC EFFECT OF OLIVE MILL WASTEWATER IN MICE INVOLVES THE SGLT-1 TRANSPORTER
Author(s) -
Nassima Senani Oularbi,
Farida Moulti Mati,
Philippe Lettéron,
Robert Ducroc,
Jamel El Benna,
J Marie
Publication year - 2016
Publication title -
international journal of pharmacy and pharmaceutical sciences/international journal of pharmacy and pharmaceutical sciences
Language(s) - English
Resource type - Journals
eISSN - 2656-0097
pISSN - 0975-1491
DOI - 10.22159/ijpps.2017v9i1.15190
Subject(s) - medicine , olive oil , chemistry , glucose transporter , endocrinology , transporter , western blot , protein kinase c , glucose uptake , insulin , pharmacology , kinase , biochemistry , food science , gene
Objective: Olive mill wastewater (OMWW) is a by-product of olive oil production and contains biologically active compounds. The aim of this study was to investigate the effect of OMWW on intestinal glucose absorption in mice, a target for treating diabetes. Methods: The effect oral administration of OMWW on mice glycemia was evaluated by oral glucose tolerance tests (OGTT), and mice jejunal mucosal strips were mounted in Ussing chambers to evaluate the activity of sodium-dependent glucose transporter (SGLT-1). Western blot analysis was performed to study SGLT-1 and protein kinase C (PKC) at the brush border membranes (BBM) of mice jejunal mucosa. Results: We found that the raised glycemia in mice in response to oral glucose administration (OGTT) was decreased in presence of OMWW, being highly effective at 4 g/kg of body weight. Further, OMWW inhibited glucose induced short-circuit current (Isc) in mice jejunal, which is a marker of SGLT-1 activity. The effect was rapid, with maximal inhibition of 80 % versus control. Also, OMWW induced a strong decrease of SGLT-1 protein (60%) as well as PKC (30%), a key effector protein in BBM of mice jejunal mucosa in response to glucose. Conclusion: Our results suggest that OMWW may be beneficial for diabetes through its novel hypoglycemic effect by acting on the PKC-SGLT-1 axis.