Open AccessCARDIOPROTECTIVE EFFECT OF MENTHA LONGIFOLIA AGAINST CYCLOPHOSPHAMIDE INDUCED CARDIOTOXICITY IN RATS: A BIOCHEMICAL, ELECTROCARDIOGRAPHIC AND HISTOPATHOLOGICAL STUDY
Author(s) -
Ayshath Afroos Shahana A.r.,
Sanjiv Karale,
Jagadish V Kamath
Publication year - 2016
Publication title -
international journal of pharmacy and pharmaceutical sciences/international journal of pharmacy and pharmaceutical sciences
Language(s) - English
Resource type - Journals
eISSN - 2656-0097
pISSN - 0975-1491
DOI - 10.22159/ijpps.2016v8i9.13004
Subject(s) - cardiotoxicity , lactate dehydrogenase , cyclophosphamide , superoxide dismutase , creatine kinase , pharmacology , antioxidant , lipid peroxidation , catalase , chemistry , endocrinology , medicine , toxicity , biochemistry , enzyme , chemotherapy
Objective: The current research was designed to evaluate the cardioprotective activity of Mentha longifolia (ML) leaf extract on cyclophosphamide-induced cardiotoxicity in rats. Methods: Cardiotoxicity was induced in Albino wistar rats of either sex by administering a single injection of cyclophosphamide (200 mg/kg, i. p.) on the first day of the experimental period. Mentha longifolia (250 and 500 mg/kg, p. o.) was administered daily for 10 d immediately after administration of cyclophosphamide on the first day. The general observations such as oxidative marker enzyme assays, ECG and histopathology were examined. Results: Cyclophosphamide administration significantly (p<0.05) increased lipid peroxidation (LPO) and decreased the levels of antioxidant markers such as superoxide dismutase (SOD) and catalase (CAT). Cyclophosphamide elevated the levels of biomarker enzymes like creatine kinase isoenzyme MB (CK-MB), creatine kinase isoenzyme NAC (CK-NAC) and lactate dehydrogenase (LDH). Further, the cyclophosphamide-treated rats showed changes in electrocardiographic parameters. Treatment with Mentha longifolia significantly (p<0.05) reversed the status of cardiac biomarkers, ECG and oxidative enzymes in cyclophosphamide-induced cardiotoxicity. Histopathological examination was also supported the potential cardioprotective effect of Mentha longifolia with reduced damage to the myocardium. Conclusion: The biochemical, ECG and histopathology reports support the potential benefits of Mentha longifolia against myocardial damage which could be attributed to antioxidant activity.