Open AccessEFFECT OF ETHANOL-WATER COMPOSITION ON CLINDAMYCIN HYDROCHLORIDE PSEUDOPOLYMORPHISM
Author(s) -
Ilma Nugrahani,
Silvana Anggraeni
Publication year - 2016
Publication title -
international journal of pharmacy and pharmaceutical sciences/international journal of pharmacy and pharmaceutical sciences
Language(s) - English
Resource type - Journals
eISSN - 2656-0097
pISSN - 0975-1491
DOI - 10.22159/ijpps.2016v8i11.14132
Subject(s) - ethanol , hydrate , chemistry , dehydration , nuclear chemistry , recrystallization (geology) , diffractometer , differential scanning calorimetry , solvent , organic chemistry , crystal structure , biochemistry , paleontology , physics , biology , thermodynamics
Objective: Formation of clindamycin hydrochloride (clindamycin HCl) in monohydrate-ethanolate from the recrystallization process with ethanol–water (5:2) has been reported a long time ago. However, the effect of ethanol-water compositions into pseudo-polymorphism formation and its stability of was not reported yet. This study aimed to investigate the effect of ethanol-water proportion on the formation of clindamycin HCl-monohydrate and its ethanol solvate.Methods: Clindamycin HCl was recrystallized with the various percentages of ethanol. The fresh and after storage for 24 h at humidity and room temperature (25±2 °C, RH: 70±1%) crystals were characterized by FTIR (Fourier transform infra-red), PXRD (powder x-ray diffractometer), and DTA (differential scanning calorimeter). The study of desolvation/dehydration then was observed with a polarization microscopy-plate heater.Results: The results showed that monohydrate crystal was obtained from recrystallization in a concentration less than 50% ethanol in water. Next, the ethanolate was produced from the solvent of>70% ethanol. Meanwhile, the 50–70 % ethanol produced a hydrate–ethanolate, crystal, which has both hydrate and ethanol in its lattice. This hydrate-ethanolates was unstable, even in ambient temperature.Conclusion: Concentration of ethanol in water as the solvent will determine the clindamycin HCl pseudo polymorphism, which will back to its original crystal form by the time of storage.