Open AccessOPTIMIZATION OF EXTRACTION PARAMETERS FOR TOTAL FLAVONOIDS FROM GARDENIA GUMMIFERA GUM RESIN BY RESPONSE SURFACE METHODOLOGY
Author(s) -
Snehal B. Bhandare,
K. S. Laddha
Publication year - 2016
Publication title -
international journal of pharmacy and pharmaceutical sciences/international journal of pharmacy and pharmaceutical sciences
Language(s) - English
Resource type - Journals
eISSN - 2656-0097
pISSN - 0975-1491
DOI - 10.22159/ijpps.2016v8i11.11134
Subject(s) - response surface methodology , extraction (chemistry) , chromatography , acetone , box–behnken design , chemistry , flavonoid , yield (engineering) , materials science , organic chemistry , metallurgy , antioxidant
Objective: To optimize the extraction parameters for determining the highest yield of Total Flavonoids from Gardenia gummifera gum resin.Methods: In the present study, response surface methodology (RSM) with three level Box Behenken design (BBD) was performed to optimize extraction parameters for total flavonoids. Solvent concentration(A), extraction time (B) and extraction temperature(C) were considered for single factor experiment.Results: The highest flavonoid concentration was obtained with acetone 45.00% v/v, time 101.46 min, temperature 41.57 °C. The average experimental TFC under optimal conditions was 161.14 mg/g which was in close agreement with the predicted value of 163.42 mg/g.Conclusion: RSM-BBD is successfully applied in optimizing extraction conditions for extraction of total flavonoids from G. gummifera gum resin.