Open AccessANTI-DIABETIC AND ALDOSE REDUCTASE INHIBITORY POTENTIAL OF PSIDIUM GUAJAVA BY IN VITRO ANALYSIS
Author(s) -
Suchith Anand,
Munichetty Arasakumari,
Panneervelu Prabu,
Arul Joseph Amalraj
Publication year - 2016
Publication title -
international journal of pharmacy and pharmaceutical sciences/international journal of pharmacy and pharmaceutical sciences
Language(s) - English
Resource type - Journals
eISSN - 2656-0097
pISSN - 0975-1491
DOI - 10.22159/ijpps.2016.v8i9.13532
Subject(s) - aldose reductase , chemistry , psidium , wortmannin , ic50 , inhibitory postsynaptic potential , in vitro , sorbitol , biochemistry , enzyme , endocrinology , biology , pi3k/akt/mtor pathway , apoptosis , botany
Objective: The objective of the present study was to determine the cellular level effect on glucose uptake and aldose reductase inhibitory activity of different extracts of traditional medicinal plant Psidium guajava . Methods: Psidium guajava was selected and subjected for successive extraction from non-polar to polar solvents and subjected to glucose uptake and aldose reductase inhibition assay. Results: Based on the results Psidium guajava methanolic extract (PGME) showed an enhancement in the glucose uptake and also up-regulates the gene and protein level expression of IRβ, IRS-1, PI3K and GLUT4. Wortmannin, a specific PI3K inhibitor confirms that the active PGME recruits glucose uptake through a PI3K dependent pathway. In the assay of aldose reductase inhibitory activity, the results suggested that PGME possesses a significant inhibitory effect. Conclusion: The result obtained in the present study focuses on the anti-diabetic effect of PGME by studying cellular level glucose uptake in L6 myotubes and aldose reductase inhibitory activity.