Open AccessDEVELOPMENT AND VALIDATION OF UV-VISIBLE SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF TADALAFIL IN BULK AND FORMULATION
Author(s) -
Shrishail M. Ghurghure,
Manisha S. Dyawarkonda,
Sachin Yanjane
Publication year - 2020
Publication title -
international journal of current pharmaceutical research
Language(s) - English
Resource type - Journals
ISSN - 0975-7066
DOI - 10.22159/ijcpr.2020v12i3.38310
Subject(s) - tadalafil , linearity , dimethyl sulfoxide , chemistry , robustness (evolution) , chromatography , linear regression , analytical chemistry (journal) , mathematics , statistics , organic chemistry , engineering , electronic engineering , medicine , biochemistry , sildenafil , gene
Objective: A new simple, sensitive, precise and reproducible spectroscopic method was developed for the determination of Tadalafil in Pharmaceutical formulation with Dimethyl Sulfoxide.
Methods: The UV spectrum of Tadalafil in Dimethyl sulfoxide (DMSO) showed λ max at 285.6 nm. Beer’s law is valid in the concentration range of 10-60µg/ml. This method was validated for linearity, accuracy, precision, ruggedness and robustness.
Results: The method was demonstrated excellent linearity over the range of 10-60µg/ml with regression equation y= 0.0337x-0.1343 and regression correlation R2= 0.998. Moreover, the method was found to be highly sensitive with LOD (2.44µg/ml) and LOQ (7.40µg/ml).
Conclusion: Based on results, the proposed method can be successfully applied for assay of Tadalafil in various pharmaceutical dosage forms.