Open AccessSPECTROPHOTOMETRIC DETERMINATION OF DASATINIB IN PHARMACEUTICAL FORMULATIONS
Author(s) -
Yogesh Thorat,
Anita Shegaonkar,
Smita Kumbhar,
Vinod Matole,
Avinash H. Hosmani
Publication year - 2020
Publication title -
international journal of current pharmaceutical research
Language(s) - English
Resource type - Journals
ISSN - 0975-7066
DOI - 10.22159/ijcpr.2020v12i2.37492
Subject(s) - dasatinib , bioanalysis , chromatography , chloranilic acid , chemistry , linearity , linear regression , dosage form , correlation coefficient , pharmaceutical formulation , analytical chemistry (journal) , mathematics , statistics , physics , biochemistry , signal transduction , tyrosine kinase , quantum mechanics
Objective: A new, simple, sensitive, precise and reproducible bioanalytical method was developed for the determination of Dasatinib in pharmaceutical formulations with Chloranilic acid.
Methods: The method is based on formation of violet colored complex. The UV spectrum of Dasatinib in methanol showed λ max at 521 nm. Beer’s law is valid in the concentration range of 10-60 μg/ml. This method was validated for linearity, accuracy, precision, ruggedness and robustness.
Results: The method has demonstrated excellent linearity over the range of 10-60 μg/ml with regression equation y = 0.021x-0.083 and regression correlation coefficient r2= 0.997. Moreover, the method was found to be highly sensitive with LOD (2.96μg/ml) and LOQ (8.98μg/ml).
Conclusion: Based on results the proposed method can be successfully applied for the assay of Dasatinib in various pharmaceutical dosageforms.