Open AccessCYTOTOXICITY OF AROMATIC COMPOUND FROM AN ENDOPHYTIC FUNGUS, CLADOSPORIUM SP. EN-S01
Author(s) -
R. R. Indry Noviarin Examinati,
Asri Peni Wulandari,
Desi Harneti Putri Huspa,
Poniah Andayaningsih
Publication year - 2018
Publication title -
international journal of current pharmaceutical research
Language(s) - English
Resource type - Journals
ISSN - 0975-7066
DOI - 10.22159/ijcpr.2018v10i6.30964
Subject(s) - cytotoxicity , cladosporium , ethyl acetate , plant use of endophytic fungi in defense , chemistry , fungus , mtt assay , ic50 , mcf 7 , chromatography , in vitro , stereochemistry , botany , food science , biology , biochemistry , cancer cell , cancer , human breast , penicillium , genetics
Objective: Endophytes have the potential to synthesize various bioactive secondary metabolites. The aims of the study were to isolate the major compound from endophytic Cladosporium sp. EN-S01., identify its function group, then evaluate its cytotoxicity.Methods: The endophytic fungus was grown in potato dextrose broth and extracted using ethyl acetate. Secondary metabolites were isolated by chromatographic separation and re-crystallization, and function group was determined by infrared spectroscopy. In vitro cytotoxicity was evaluated by the prestoblue assay.Result: The isolated aromatic compound evaluated by prestoblue assay against breast cancer cell line MCF-7 showed cytotoxicity with IC50 value of 746,03 ppm.Conclusion: Our findings indicate this aromatic compound might be useful lead compounds to develop cytotoxic drugs.